Composite
Part:BBa_K2481106
Designed by: Erin Kelly Group: iGEM17_Lethbridge_HS (2017-10-27)
indB expression construct
indB is a putative phosphatase from Streptomyces crhomofuscus whose native function has not been well characterized. It has been shown that expression of IndB in conjuction with the indogoidine sythase indC will increase indigoidine yields. This part allows for the ITPG inducible epxression of IndB in E. coli BL21(DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 701
Illegal BamHI site found at 852 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1135
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |